Space & Astronomy
28 min read
Understanding Carbon Flux Allocation in Cassava Cultivars
Nature
January 19, 2026•3 days ago
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Cassava cultivars exhibit variations in carbon flux allocation due to a balanced competition between starch accumulation and structural component development. This competition influences how carbon is distributed within the plant, impacting yield potential. Differences in lignin content are correlated with starch accumulation, with this relationship varying across different cassava varieties.
Bredeson, J. V. et al. Sequencing wild and cultivated cassava and related species reveals extensive interspecific hybridization and genetic diversity. Nat. Biotechnol. 34, 562–570 (2016).
El-Sharkawy, M. A. Cassava biology and physiology. Plant Mol. Biol. 56, 481–501 (2004).
Latif, S. & Müller, J. Potential of cassava leaves in human nutrition: A review. Trends Food Sci. Technol. 44, 147–158 (2015).
Cai, Z. et al. Impact of lignin on the starch accumulation, composition, and pasting properties of cassava. LWT 184, 115073 (2023).
Sun, J., Hui, K., Guo, Z., Li, Y. & Fan, X. Cellulose and Lignin Contents are Negatively Correlated with Starch Accumulation, and Their Correlation Characteristics Vary Across Cassava Varieties. J. Plant Growth Regul. 42, 658–669 (2023).
Singh, V. et al. Proximal and distal parts of sweetpotato adventitious roots display differences in root architecture, lignin, and starch metabolism and their developmental fates. Front. Plant Sci. 11, 609923 (2021).
Pan, K. et al. Predominantly symplastic phloem unloading of photosynthates maintains efficient starch accumulation in the cassava storage roots (Manihot esculenta Crantz). BMC Plant Biol. 21, 318 (2021).
De Souza, A. P. et al. Rooting for cassava: insights into photosynthesis and associated physiology as a route to improve yield potential. New Phytol. 213, 50–65 (2017).
De Souza, A. P., Wang, Y., Orr, D. J., Carmo-Silva, E. & Long, S. P. Photosynthesis across African cassava germplasm is limited by Rubisco and mesophyll conductance at steady state, but by stomatal conductance in fluctuating light. New Phytol. 225, 2498–2512 (2020).
An, F. et al. Protein Cross-Interactions for Efficient Photosynthesis in the Cassava Cultivar SC205 Relative to Its Wild Species. J. Agric. Food Chem. 67, 8746–8755 (2019).
Braun, D. M. Phloem Loading and Unloading of Sucrose: What a Long, Strange Trip from Source to Sink. Annual Review of Plant Biology 73, 553–584 (2022).
Szydlowski, N. et al. Integrated functions among multiple starch synthases determine both amylopectin chain length and branch linkage location in Arabidopsis leaf starch. J. Exp. Bot. 62, 4547–4559 (2011).
Mehdi, R. et al. Symplasmic phloem unloading and radial post-phloem transport via vascular rays in tuberous roots of Manihot esculenta. J. Exp. Bot. 70, 5559–5573 (2019).
Rüscher, D. et al. Symplasmic phloem loading and subcellular transport in storage roots are key factors for carbon allocation in cassava. Plant Physiol. 196, 1322–1339 (2024).
He, Y. et al. Sugar Metabolism and Transcriptome Analysis Reveal Key Sugar Transporters during Camellia oleifera Fruit Development. Int. J. Mol. Sci. 23, 822 (2022).
Lloyd, J. R. & Kossmann, J. Starch trek: The search for yield. Front. Plant Sci. 9, 1–8 (2019).
Utsumi, Y. et al. Integrative omics approaches revealed a crosstalk among phytohormones during tuberous root development in cassava. Plant Mol. Biol. 109, 249–269 (2022).
Vanholme, R., De Meester, B., Ralph, J. & Boerjan, W. Lignin biosynthesis and its integration into metabolism. Curr. Opin. Biotechnol. 56, 230–239 (2019).
Singh, V. et al. Gibberellin Promotes Sweetpotato Root Vascular Lignification and Reduces Storage-Root Formation. Front. Plant Sci. 10, 1–22 (2019).
Maeda, H. & Dudareva, N. The shikimate pathway and aromatic amino acid biosynthesis in plants. Annu. Rev. Plant Biol. 63, 73–105 (2012).
Jiao, Y. et al. Transcriptome analysis provides new ideas for studying the regulation of glucose-induced lignin biosynthesis in pear calli. BMC Plant Biol. 22, 1–18 (2022).
Wang, H. et al. Altered Phenylpropanoid Metabolism in the Maize Lc-Expressed Sweet Potato (Ipomoea batatas) Affects Storage Root Development. Sci. Rep. 6, 1–15 (2016).
Wahl, S. A., Dauner, M. & Wiechert, W. New Tools for Mass Isotopomer Data Evaluation in 13C Flux Analysis: Mass Isotope Correction, Data Consistency Checking, and Precursor Relationships. Biotechnol. Bioeng. 85, 259–268 (2004).
Heise, R. et al. Flux profiling of photosynthetic carbon metabolism in intact plants. Nat. Protoc. 9, 1803–1824 (2014).
Uy, A. L. T. et al. The Carbon Flow Shifts from Primary to Secondary Metabolism during Xylem Vessel Cell Differentiation in Arabidopsis thaliana. Plant Cell Physiol. 64, 1563–1575 (2023).
López-Calcagno, P. E. et al. Overexpressing the H-protein of the glycine cleavage system increases biomass yield in glasshouse and field-grown transgenic tobacco plants. Plant Biotechnol. J. 17, 141–151 (2019).
Makino, A., Suzuki, Y. & Ishiyama, K. Enhancing photosynthesis and yield in rice with improved N use efficiency. Plant Sci. 325, 111475 (2022).
El-Sharkawy, M. A. & De Tafur, S. M. Genotypic and within canopy variation in leaf carbon isotope discrimination and its relation to short-term leaf gas exchange characteristics in cassava grown under rain-fed conditions in the tropics. Photosynthetica 45, 515–526 (2007).
Fan, X. W. et al. MeSWEET15a/b genes play a role in the resistance of cassava (Manihot esculenta Crantz) to water and salt stress by modulating sugar distribution. Plant Physiol. Biochem. 194, 394–405 (2023).
Büttner, M. The Arabidopsis sugar transporter (AtSTP) family: An update. Plant Biol. 12, 35–41 (2010).
Salvi, P. et al. Sugar transporters and their molecular tradeoffs during abiotic stress responses in plants. Physiol. Plant. 174, e13652 (2022).
Zhong, Y. et al. Transport and spatio-temporal conversion of sugar facilitate the formation of spatial gradients of starch in wheat caryopses. Commun. Biol. 7, 1–11 (2024).
Narnoliya, L. K., Sangwan, N., Jadaun, J. S., Bansal, S. & Sangwan, R. S. Defining the role of a caffeic acid 3-O-methyltransferase from Azadirachta indica fruits in the biosynthesis of ferulic acid through heterologous over-expression in Ocimum species and Withania somnifera. Planta 253, 1–13 (2021).
Yan, W. et al. Cell wall invertase 3 affects cassava productivity via regulating sugar allocation from source to sink. Front. Plant Sci. 10, 1–16 (2019).
Rog, I., Jakoby, G. & Klein, T. Carbon allocation dynamics in conifers and broadleaved tree species revealed by pulse labeling and mass balance. For. Ecol. Manage. 493, 119258 (2021).
Desalme, D. et al. Seasonal variations drive short-term dynamics and partitioning of recently assimilated carbon in the foliage of adult beech and pine. New Phytol. 213, 140–153 (2017).
Tcherkez, G., Mahé, A. & Hodges, M. 12C/13C fractionations in plant primary metabolism. Trends Plant Sci. 16, 499–506 (2011).
Baroja-Fernández, E. et al. Enhancing sucrose synthase activity in transgenic potato (Solanum tuberosum L.) tubers results in increased levels of starch, ADPglucose and UDPglucose and total yield. Plant Cell Physiol. 50, 1651–1662 (2009).
Yu, T., Lue, W., Wang, S. & Chen, J. Mutation of Arabidopsis Plastid Phosphoglucose Isomerase Affects Leaf Starch Synthesis and Floral Initiation. Plant Physiol. 123, 319–325 (2000).
Granot, D. Role of tomato hexose kinases. Funct. Plant Biol. 34, 564–570 (2007).
Granot, D., David-Schwartz, R. & Kelly, G. Hexose kinases and their role in sugar-sensing and plant development. Front. Plant Sci. 4, 1–18 (2013).
Adly, W. M. R. M. et al. Somaclonal Variation for Genetic Improvement of Starch Accumulation in Potato (Solanum tuberosum) Tubers. Plants 12, 1–14 (2023).
Weber, H. et al. Antisense-inhibition of ADP-glucose pyrophosphorylase in developing seeds of Vicia narbonensis moderately decreases starch but increases protein content and affects seed maturation. Plant J. 24, 33–43 (2000).
Yelle, S., Hewitt, J. D., Robinson, N. L., Damon, S. & Bennett, A. B. Sink metabolism in tomato fruit: III. Analysis of carbohydrate assimilation in a wild species. Plant Physiol. 87, 737–740 (1988).
Beyene, G., Chauhan, R. D., Gehan, J., Siritunga, D. & Taylor, N. Cassava shrunken-2 homolog MeAPL3 determines storage root starch and dry matter content and modulates storage root postharvest physiological deterioration. Plant Mol. Biol. 109, 283–299 (2022).
Boerjan, W., Ralph, J. & Baucher, M. Lignin biosynthesis. Annu. Rev. Plant Biol. 54, 519–546 (2003).
Barros, J. et al. 4-Coumarate 3-hydroxylase in the lignin biosynthesis pathway is a cytosolic ascorbate peroxidase. Nat. Commun. 10, 1–11 (2019).
Vanholme, R. et al. A systems biology view of responses to lignin biosynthesis perturbations in Arabidopsis. Plant Cell 24, 3506–3529 (2012).
Guo, D., Chen, F., Inoue, K., Blount, J. W. & Dixon, R. A. Downregulation of caffeic acid 3-O-methyltransferase and caffeoyl CoA 3-O-methyltransferase in transgenic alfalfa: Impacts on lignin structure and implications for the biosynthesis of G and S lignin. Plant Cell 13, 73–88 (2001).
Dominguez, P. G. et al. Sucrose synthase determines carbon allocation in developing wood and alters carbon flow at the whole tree level in aspen. New Phytol. 229, 186–198 (2021).
Chu, K. L. et al. Metabolic flux analysis of the non-transitory starch tradeoff for lipid production in mature tobacco leaves. Metab. Eng. 69, 231–248 (2022).
Amir, J. & Preiss, J. Kinetic characterization of spinach leaf sucrose-phosphate synthase. Plant Physiol. 69, 1027–1030 (1982).
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